Sunday, July 3, 2022
HomeHealthFlu virus shells could improve delivery of mRNA into cells - Phys.org

Flu virus shells could improve delivery of mRNA into cells – Phys.org


Forget Password?
Learn more
share this!
589
82
Share
Email
November 30, 2021
by
Nanoengineers at the University of California San Diego have developed a new and potentially more effective way to deliver messenger RNA (mRNA) into cells. Their approach involves packing mRNA inside nanoparticles that mimic the flu virus—a naturally efficient vehicle for delivering genetic material such as RNA inside cells.

The new mRNA nanoparticles are described in a paper published recently in the journal Angewandte Chemie International Edition.
The work addresses a major challenge in the field of drug delivery: Getting large biological drug molecules safely into and protecting them from organelles called endosomes. These tiny acid-filled bubbles inside the cell serve as barriers that trap and digest large molecules that try to enter. In order for biological therapeutics to do their job once they are inside the cell, they need a way to escape the endosomes.
“Current mRNA delivery methods do not have very effective endosomal escape mechanisms, so the amount of mRNA that actually gets released into cells and shows effect is very low. The majority of them are wasted when they get administered,” said senior author Liangfang Zhang, a professor of nanoengineering at the UC San Diego Jacobs School of Engineering.
Achieving efficient endosomal escape would be a game changer for mRNA vaccines and therapies, explained Zhang. “If you can get more mRNA into cells, this means you can take a much lower dose of an mRNA vaccine, and this could reduce side effects while achieving the same efficacy.” It could also improve delivery of small interfering RNA (siRNA) into cells, which is used in some forms of gene therapy.
In nature, viruses do a very good job of escaping the endosome. The influenza A virus, for example, has a special protein on its surface called hemagglutinin, that when activated by acid inside the endosome, triggers the virus to fuse its membrane with the endosomal membrane. This opens up the endosome, enabling the virus to release its into the host cell without getting destroyed.
Zhang and his team developed mRNA delivery nanoparticles that mimic the flu virus’s ability to do this. To make the nanoparticles, the researchers genetically engineered cells in the lab to express the hemagglutinin protein on their cell membranes. They then separated the membranes from the cells, broke them into tiny pieces, and coated them onto nanoparticles made from a biodegradable polymer that has been pre-packed with mRNA molecules inside.
The finished product is a flu virus-like nanoparticle that can get into a cell, break out of the endosome, and free its mRNA payload to do its job: Instruct the cell to produce proteins.
The researchers tested the nanoparticles in mice. The nanoparticles were packed with mRNA encoding for a bioluminescent protein called Cypridina luciferase. They were administered both through the nose—the mice inhaled droplets of a nanoparticle-containing solution applied at the nostrils—and via intravenous injection. The researchers imaged the noses and assayed the blood of the mice and found a significant amount of bioluminescence signal. This was evidence that the -like effectively delivered their mRNA payloads into cells in vivo.
The researchers are now testing their system for delivery of therapeutic mRNA and siRNA payloads.


Explore further

Researchers monitor mRNA to help time its great escape to perfection


More information: Joon Ho Park et al, Virus‐Mimicking Cell Membrane‐Coated Nanoparticles for Cytosolic Delivery of mRNA, Angewandte Chemie International Edition (2021). DOI: 10.1002/anie.202113671

Citation: Flu virus shells could improve delivery of mRNA into cells (2021, November 30) retrieved 18 May 2022 from https://phys.org/news/2021-11-flu-virus-shells-delivery-mrna.html
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.

Explore further
Facebook
Twitter
Email
Feedback to editors
15 hours ago
0
17 hours ago
0
17 hours ago
0
17 hours ago
0
May 16, 2022
1
2 hours ago
8 hours ago
8 hours ago
8 hours ago
8 hours ago
9 hours ago
9 hours ago
7 hours ago
7 hours ago
7 hours ago
7 hours ago
7 hours ago
8 hours ago
More from Physics Forums | Science Articles, Homework Help, Discussion
Jun 09, 2021
Feb 17, 2021
Oct 15, 2021
Aug 12, 2021
Sep 28, 2021
Oct 28, 2021
15 hours ago
May 16, 2022
May 16, 2022
May 12, 2022
May 12, 2022
May 12, 2022
Use this form if you have come across a typo, inaccuracy or would like to send an edit request for the content on this page. For general inquiries, please use our contact form. For general feedback, use the public comments section below (please adhere to guidelines).
Please select the most appropriate category to facilitate processing of your request
Thank you for taking time to provide your feedback to the editors.
Your feedback is important to us. However, we do not guarantee individual replies due to the high volume of messages.
Your email address is used only to let the recipient know who sent the email. Neither your address nor the recipient’s address will be used for any other purpose. The information you enter will appear in your e-mail message and is not retained by Phys.org in any form.

Get weekly and/or daily updates delivered to your inbox. You can unsubscribe at any time and we’ll never share your details to third parties.
More information Privacy policy
Medical research advances and health news
The latest engineering, electronics and technology advances
The most comprehensive sci-tech news coverage on the web
This site uses cookies to assist with navigation, analyse your use of our services, collect data for ads personalisation and provide content from third parties. By using our site, you acknowledge that you have read and understand our Privacy Policy and Terms of Use.

source

RELATED ARTICLES

LEAVE A REPLY

Please enter your comment!
Please enter your name here

Most Popular

Recent Comments